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mouse trim55  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology mouse trim55
    Mouse Trim55, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/murf2/MuRF2+siRNA/pm37499037-341-28-42
    Average 91 stars, based on 1 article reviews
    mouse trim55 - by Bioz Stars, 2026-11
    91/100 stars

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    Related Articles

    Western Blot:

    Article Title: MuRF1 and MuRF2 are key players in skeletal muscle regeneration involving myogenic deficit and deregulation of the chromatin-remodeling complex
    Article Snippet: .. The primary antibodies used for western blot were: (1) MuRF1 and (2) MuRF2 [27, 32]; (2) Atrogin/Mafbx‐1 (Cat#AP2041, ECM Biosciences); (3) Myf‐5 (Cat#sc‐302, Santa Cruz); (4) Myogenin (Cat#ab1835, Abcam); (5) sarcomeric actin (Cat#M0847, Dako); (6) GAPDH (Cat# 2118, CellSignaling), (7) FHL2 (Cat# A300‐332A, Bethyl Laboratories); (8) MARP2; (9) BAF57 (Cat#Abd52, Millipore). .. The secondary antibodies used for western blot were: (1) Donkey anti‐Chicken (Jackson ImmunoResearch); (2) Goat anti‐Rabbit (Jackson ImmunoResearch); (3) Goat anti‐Mouse (Jackson ImmunoResearch).

    other:

    Article Title: SIRT1 may play a crucial role in overload‐induced hypertrophy of skeletal muscle
    Article Snippet: The membranes were subsequently blocked in 1–5% skimmed milk or BSA, and after blocking, PVDF membranes were incubated at room temperature with primary antibodies [Nampt 1:500 Abcam (Cambridge, MA, USA) no. ab37299, SIRT1 1:3000 Abcam no. ab110304, Acetyl‐p53 (Lys373, Lys382) 1:1000 Millipore (Billerica, MA, USA) no. 06‐758, Akt 1:1000 Cell Signaling (Danvers, MA, USA) no. 9272, Phospho‐Akt (Ser473) 1:1000 Cell Signaling no. 9271, IR‐beta 1:1000 Santa Cruz Biotechnology (Santa Cruz, CA, USA) no. sc‐711, Myf5 1:5000 Abcam no. ab125078, MyoD 1:1000 Abcam no. ab126726, PCNA 1:500 Santa Cruz no. sc‐7907, eNOS 1:625 Abcam no. 76198, VEGF 1:1000 Santa Cruz no. sc‐152, GLUT4 1:200 Santa Cruz no. sc‐7938, FOXO1 1:1000 Cell Signaling no. 9454, K48‐linkage Specific Polyubiquitin 1:1000 Cell Signaling no. 8081S, K63‐linkage Specific Polyubiquitin 1:1000 Cell Signaling no. 5621S, MuRF1 1:1000 Santa Cruz no. sc‐32920, MuRF2 1:500 Santa Cruz no. sc‐49457, Proteasome subunit alpha type‐6 (PSMA6) 1:1200 Cell Signaling no. 2459S, Bax 1:500 Santa Cruz no. 492, glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) 1:10 000 Sigma‐Aldrich (St Louis, MO, USA) no. G8795].



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    <t>TRIM55</t> is downregulated in HCC tissues and is associated with prognosis of HCC patients. ( A ) IHC detected expression of TRIM55 in HCC tissues and neighboring tissues. Representative photos at 200× and 400×. The level of TRIM55 expression between HCC tissues and neighbor tissues was analyzed and shown as a pie chart. ( B, C ) the relationship between overall survival and TRIM55 expression was analyzed by Kaplan-Meier analysis, and the follow-up data were collected by ourselves ( B ) or TCGA database ( C ).
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    Image Search Results


    Western blotting analysis of proteins involved in the regulation of skeletal muscle mass and function. ( A ) Representative bands of each protein assessed by Western blot (MW = molecular weight). ( B – D ) Densitometry of total HDAC4, phospho (Ser632) HDAC4, and the ratio of phospho/total HDAC4 protein levels. ( E ) Densitometry of MuRF1 protein levels. ( F ) Densitometry of MuRF2 protein levels. ( G – I ) Densitometry of total FoxO1, phospho (Ser256) FoxO1, and the ratio of phospho/total FoxO1 protein levels. ( J – L ) Densitometry of total Akt, phospho (Ser473) Akt, and the ratio of phospho/total Akt protein levels. Data are shown as mean ± standard error of the mean (* p < 0.05 vs. Sham12h, # p < 0.05 vs. DNV12h + VEH, n = 5–7).

    Journal: International Journal of Molecular Sciences

    Article Title: Small-Molecule Inhibition of MuRF1 Prevents Early Disuse-Induced Diaphragmatic Dysfunction and Atrophy

    doi: 10.3390/ijms24043637

    Figure Lengend Snippet: Western blotting analysis of proteins involved in the regulation of skeletal muscle mass and function. ( A ) Representative bands of each protein assessed by Western blot (MW = molecular weight). ( B – D ) Densitometry of total HDAC4, phospho (Ser632) HDAC4, and the ratio of phospho/total HDAC4 protein levels. ( E ) Densitometry of MuRF1 protein levels. ( F ) Densitometry of MuRF2 protein levels. ( G – I ) Densitometry of total FoxO1, phospho (Ser256) FoxO1, and the ratio of phospho/total FoxO1 protein levels. ( J – L ) Densitometry of total Akt, phospho (Ser473) Akt, and the ratio of phospho/total Akt protein levels. Data are shown as mean ± standard error of the mean (* p < 0.05 vs. Sham12h, # p < 0.05 vs. DNV12h + VEH, n = 5–7).

    Article Snippet: The following primary antibodies were used: rabbit anti-4-HNE (1:1000, Sigma Aldrich, #393207, St. Louis, MO, USA), rabbit anti-HDAC4 (1:1000, Cell Signaling, #7628, Danvers, MA, USA), rabbit anti-phospho(Ser632) HDAC4 (1:1000; Sigma Aldrich, #SAB4300155), rabbit anti-FoxO1 (1:1000, Cell Signaling, #2880), rabbit anti-phospho(Ser256) FoxO1 (1:000, Cell Signaling, #9461), rabbit anti-Akt (1:000, Cell Signaling, #9272), rabbit anti-phosphor(Ser473) Akt (1:000, Cell Signaling, #4058), rabbit anti-MuRF1 (1:1000, Myomedix, Neckargemünd, Germany), rabbit anti-MuRF2 (1:1000, Myomedix), rabbit anti-GAPDH (1:3000; Cell Signaling, #2118), and rabbit anti-α-tubulin (1:1000, Cell Signaling, #2125).

    Techniques: Western Blot, Molecular Weight

    TRIM55 is downregulated in HCC tissues and is associated with prognosis of HCC patients. ( A ) IHC detected expression of TRIM55 in HCC tissues and neighboring tissues. Representative photos at 200× and 400×. The level of TRIM55 expression between HCC tissues and neighbor tissues was analyzed and shown as a pie chart. ( B, C ) the relationship between overall survival and TRIM55 expression was analyzed by Kaplan-Meier analysis, and the follow-up data were collected by ourselves ( B ) or TCGA database ( C ).

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Overexpression of Tripartite Motif Conaining 55 (TRIM55) Inhibits Migration and Invasion of Hepatocellular Carcinoma (HCC) Cells via Epithelial-Mesenchymal Transition and Matrix Metalloproteinase-2 (MMP2)

    doi: 10.12659/MSM.910984

    Figure Lengend Snippet: TRIM55 is downregulated in HCC tissues and is associated with prognosis of HCC patients. ( A ) IHC detected expression of TRIM55 in HCC tissues and neighboring tissues. Representative photos at 200× and 400×. The level of TRIM55 expression between HCC tissues and neighbor tissues was analyzed and shown as a pie chart. ( B, C ) the relationship between overall survival and TRIM55 expression was analyzed by Kaplan-Meier analysis, and the follow-up data were collected by ourselves ( B ) or TCGA database ( C ).

    Article Snippet: The cDNA clone of TRIM55 was obtained from Origene (Cat. No. RG214960), then we constructed the transient overexpression of TRIM55 in Huh7 and HCC-LM3 cell lines according to the product manual.

    Techniques: Expressing

    Relationship between TRIM55 expression and clinicopathologic features.

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Overexpression of Tripartite Motif Conaining 55 (TRIM55) Inhibits Migration and Invasion of Hepatocellular Carcinoma (HCC) Cells via Epithelial-Mesenchymal Transition and Matrix Metalloproteinase-2 (MMP2)

    doi: 10.12659/MSM.910984

    Figure Lengend Snippet: Relationship between TRIM55 expression and clinicopathologic features.

    Article Snippet: The cDNA clone of TRIM55 was obtained from Origene (Cat. No. RG214960), then we constructed the transient overexpression of TRIM55 in Huh7 and HCC-LM3 cell lines according to the product manual.

    Techniques: Expressing

    TRIM55 expression is an independent predictive factor for overall survival time in HCC patients.

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Overexpression of Tripartite Motif Conaining 55 (TRIM55) Inhibits Migration and Invasion of Hepatocellular Carcinoma (HCC) Cells via Epithelial-Mesenchymal Transition and Matrix Metalloproteinase-2 (MMP2)

    doi: 10.12659/MSM.910984

    Figure Lengend Snippet: TRIM55 expression is an independent predictive factor for overall survival time in HCC patients.

    Article Snippet: The cDNA clone of TRIM55 was obtained from Origene (Cat. No. RG214960), then we constructed the transient overexpression of TRIM55 in Huh7 and HCC-LM3 cell lines according to the product manual.

    Techniques: Expressing

    TRIM55 overexpression in HCC cell lines was constructed. ( A ) Western blot was used to verify transfection efficiency at the protein level. GAPDH was used as internal control. ( B ) RT-PCR was used to verify transfection efficiency at the mRNA level, *** p<0.0001.

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Overexpression of Tripartite Motif Conaining 55 (TRIM55) Inhibits Migration and Invasion of Hepatocellular Carcinoma (HCC) Cells via Epithelial-Mesenchymal Transition and Matrix Metalloproteinase-2 (MMP2)

    doi: 10.12659/MSM.910984

    Figure Lengend Snippet: TRIM55 overexpression in HCC cell lines was constructed. ( A ) Western blot was used to verify transfection efficiency at the protein level. GAPDH was used as internal control. ( B ) RT-PCR was used to verify transfection efficiency at the mRNA level, *** p<0.0001.

    Article Snippet: The cDNA clone of TRIM55 was obtained from Origene (Cat. No. RG214960), then we constructed the transient overexpression of TRIM55 in Huh7 and HCC-LM3 cell lines according to the product manual.

    Techniques: Over Expression, Construct, Western Blot, Transfection, Reverse Transcription Polymerase Chain Reaction

    Overexpression of TRIM55 inhibits migration and invasion of HCC cells. ( A, B ) Cell migration and invasion ability were detected by Transwell assay in HCC cells with TRIM55 overexpression and negative control. All experiments were repeated 3 times. *** p<0.0001

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Overexpression of Tripartite Motif Conaining 55 (TRIM55) Inhibits Migration and Invasion of Hepatocellular Carcinoma (HCC) Cells via Epithelial-Mesenchymal Transition and Matrix Metalloproteinase-2 (MMP2)

    doi: 10.12659/MSM.910984

    Figure Lengend Snippet: Overexpression of TRIM55 inhibits migration and invasion of HCC cells. ( A, B ) Cell migration and invasion ability were detected by Transwell assay in HCC cells with TRIM55 overexpression and negative control. All experiments were repeated 3 times. *** p<0.0001

    Article Snippet: The cDNA clone of TRIM55 was obtained from Origene (Cat. No. RG214960), then we constructed the transient overexpression of TRIM55 in Huh7 and HCC-LM3 cell lines according to the product manual.

    Techniques: Over Expression, Migration, Transwell Assay, Negative Control

    Overexpression of TRIM55 inhibits migration and invasion of HCC cells through EMT and MMP2. ( A ) IF was used to detect expression of E-cadherin and Vimentin in HCC cells with TRIM55 overexpression and negative control. ( B ) Western blot was used to detect expression of E-cadherin, Vimentin, and MMP2 in HCC cells with TRIM55 overexpression and negative control. GAPDH was used as internal control.

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Overexpression of Tripartite Motif Conaining 55 (TRIM55) Inhibits Migration and Invasion of Hepatocellular Carcinoma (HCC) Cells via Epithelial-Mesenchymal Transition and Matrix Metalloproteinase-2 (MMP2)

    doi: 10.12659/MSM.910984

    Figure Lengend Snippet: Overexpression of TRIM55 inhibits migration and invasion of HCC cells through EMT and MMP2. ( A ) IF was used to detect expression of E-cadherin and Vimentin in HCC cells with TRIM55 overexpression and negative control. ( B ) Western blot was used to detect expression of E-cadherin, Vimentin, and MMP2 in HCC cells with TRIM55 overexpression and negative control. GAPDH was used as internal control.

    Article Snippet: The cDNA clone of TRIM55 was obtained from Origene (Cat. No. RG214960), then we constructed the transient overexpression of TRIM55 in Huh7 and HCC-LM3 cell lines according to the product manual.

    Techniques: Over Expression, Migration, Expressing, Negative Control, Western Blot